Hydrolysis of Cyclic Guanosine and Adenosine 3',5'-Monophosphates by Rat and Bovine Tissues
نویسندگان
چکیده
منابع مشابه
Hydrolysis of cyclic guanosine and adenosine 3',5'-monophosphates by rat and bovine tissues.
An assay for measuring 3’,5’-cyclic nucleotide phosphodiesterase activity over a wide range of substrate concentrations has been described. The ratio of cyclic adenosine 3’,5’monophosphate (cyclic AMP) to cyclic guanosine 3’,5’monophosphate (cyclic GMP) hydrolysis varied several fold among crude subcellular fractions from bovine and rat heart when measured at micromolar but not millimolar subst...
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1. At least two classes of high-affinity cyclic AMP-binding proteins have been identified: those derived from cyclic AMP-dependent protein kinases (regulatory subunits) and those that bind a wide range of adenine analogues (adenine analogue-binding proteins). 2. In fresh-tissue extracts, regulatory subunits could be further subdivided into 'type I or 'type II' depending on whether they were der...
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The GTP cyclohydrolase activity of rat tissues has been studied by means of the measurement of formic acid release and neopterin synthesis from GTP. After gel filtration of a 45%-satd.-(NH4)2SO4 fraction of liver homogenates, three enzyme fractions were separated and named A1, A2 and A3 according to the order of their elution. Fractions A1 and A3 displayed an 8-formyl-GTP deformylase activity; ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62703-6